The Institute of Microbiology of the National Academy of Sciences of Belarus offers consumers recombinant enzymes «Uridine phosphorylase», «Thymidine phosphorylase», «Purine nucleoside phosphorylase» for chemical-enzymatic synthesis of pharmaceutical substances under a manufacturing agreement and is looking for partners interested in a distribution services agreement.

The Institute of Microbiology of the National Academy of Sciences of Belarus has developed recombinant enzymes «Uridine phosphorylase», «Thymidine phosphorylase», «Purine nucleoside phosphorylase» for chemical-enzymatic synthesis of pharmaceutical substances.

Nucleoside phosphorylase (EC 2.4.2) represent the canonical group of biocatalysts most often applied for enzymatic production of modified nucleosides.

There are three main kinds of nucleoside phosphorylase enzymes:
* purine nucleoside phosphorylase (PNPase), utilizing purine nitrogen bases and nucleosides as substrates,
* thymidine phosphorylase (TPase),
* uridine phosphorylase (UPase),
using pyrimidine bases and nucleosides as substrates.

Recombinant strains of microorganisms are tens of times more active than those currently in use, obtained by canonical selection methods. Replacing traditional biocatalysts with recombinant ones allows:
* refuse to use large fermentation equipment;
* reduce the consumption of nutrient media;
* reduce energy and labor costs;
* reduce cost of target biotechnological products.

2-F-adenine-arabinoside synthesis scheme based on recombinant PNPase and UPase enzymes:


Application area: pharmaceutical industry (manufacture of antiviral and anticancer preparations).

More details in the Catalog of Developments of the "Institute of Microbiology of the National Academy of Sciences of Belarus" 2023, p.31.

Link to the recombinant enzymes "Uridine phosphorylase", "Thymidine phosphorylase", "Purine nucleoside phosphorylase" on the website of the Institute of Microbiology here. (in Russian)

Information is posted in the "Album of Promising Developments of Organizations of the National Academy of Sciences of Belarus for the Real Sector of the Economy" (2023), pp.321-322.

Enzyme preparations «Uridine phosphorylase», «Thymidine phosphorylase», «Purine nucleoside phosphorylase» have a higher activity compared to world analogs, while the cost is 30..50% lower.

Import substitution of recombinant enzymes:
«Uridine Phosphorylase», «Thymidine Phosphorylase», «Purine nucleoside phosphorylase» («BIOTANG Inc.», USA);
«Uridine Phosphorylase», «Thymidine Phosphorylase», «Purine nucleoside phosphorylase» («RayBiotech, Inc.», USA);
«Uridine Phosphorylase», «Thymidine Phosphorylase», «Purine nucleoside phosphorylase» («Sigma-Aldrich-Merck», USA);
«Uridine Phosphorylase», «Thymidine phosphorylase», «Purine nucleoside phosphorylase» («Abbexa Ltd», Great Britain).

Based on the developed enzyme preparations Research and production center «KhimPharmSintez» manufactures medicines «Leikladin», «Fludarabel» and «Leikovir» against leukemia and multiple sclerosis.

ТУ BY 100289066.095-2012 (enzymatic preparation «Uridine phosphorylase»)
ТУ BY 100289066.106-2013 (enzymatic preparation «Purine nucleoside phosphorylase»)
ТУ BY 100289066.107-2013 (enzymatic preparation «Thymidine phosphorylase»)

1. Escherichia coli bacterial strain producing purine nucleoside phosphorylase: patent 13127 Rep. of Belarus / S. V. Kvach, L. A. Eroshevskaya, A. I. Zinchenko, A. V. Shakhbazov, N. A. Kartel; date published: 30.04.2010. (in Russian)
2. Escherichia coli bacterial strain producing uridine phosphorylase: patent 15563 Rep. of Belarus / S. V. Kvach, L. A. Eroshevskaya, A. I. Zinchenko, A. V. Shakhbazov, N. A. Kartel; date published: 28.02.2012. (in Russian)
3. Escherichia coli strain producing Thermus thermophiles pyrimidine nucleoside phosphorylase: patent 19553 Republic of Belarus / A. I. Beresnev, S. V. Kvach, L. A. Eroshevskaya, A. I. Zinchenko; date published: 09.07.2015. (in Russian)

The Institute of Microbiology is the leading research center of the Republic of Belarus in the field of microbiology and biotechnology. The Institute's arsenal of innovative developments includes substances for medicines and diagnostics, biopreservatives, feed and food additives, biodisinfectants, probiotics, plant protection products, microbial fertilizers, wastewater and environmental treatment technologies that are in demand by the economy, and specific recommendations for combating biodamage.

In order to develop new technologies and pilot production of biopreparations for various purposes, ensuring the possibility of implementing original technologies for deep cultivation of producer microorganisms, isolating biologically active metabolites from the culture medium, creating competitive commercial forms of biopreparations for various purposes, the Laboratory of Fermentation Processes with pilot production was organized in 2017.

The Institute has developed, implemented and certified a quality management system for compliance with the requirements of STB ISO 9001-2015 (certificate of conformity No. BY 112 05.01.077 06883), ensuring:
- improvement of the Institute's management system;
- improving the quality and competitiveness of products and services;
- increasing consumer confidence in products and the stability of their quality;
- stimulating the entry of products into new and expansion of existing sales markets;
- improving the economic performance of the Institute;
- increasing the discipline and responsibility of employees;
- simplification of the procedure for obtaining certificates of compliance with technical regulations;
- gaining an advantage in tenders and competitions;
- increasing the level of trust on the part of representatives of supervisory authorities.

Products for legal entities and wholesale buyers are presented on the Institute's website here.

Products for private consumers (Online store) here.

The Institute of Microbiology of the National Academy of Sciences of Belarus has been certified according to the quality management system for compliance with the requirements of STB ISO 9001-2015 (certificate of conformity No. BY 112 05.01.077 06883) since 2013.

Consumers interested in purchasing recombinant enzymes «Uridine phosphorylase», «Thymidine phosphorylase», «Purine nucleoside phosphorylase» for chemical-enzymatic synthesis of pharmaceutical substances under a manufacturing agreement.

Partners interested in purchasing recombinant enzymes «Uridine phosphorylase», «Thymidine phosphorylase», «Purine nucleoside phosphorylase» for chemical-enzymatic synthesis of pharmaceutical substances under a distribution services agreement.