A new platform technology uses laser assisted gold nanorods (GNRs) to warm cryopreserved zebrafish embryos, germplasm and other millimeter-sized tissues and biomaterials. Biocompatible gold nanorods absorb pulsed laser energy to generate rapid and uniform warming (up to 14,000,000 Â°C/min) in frozen biomaterials. Laser assisted GNR heating is the only method able to generate such high heating rates uniformly inside a millimeter-sized embryo, and it can also be used in cryopreserving a myriad of other germplasm, model systems, and other similar and smaller sized biomaterials into which GNR can be disbursed. This technology can positively impact translational medicine (which uses embryos as a genetic model of disease), embryology and toxicity as well as aquaculture and biodiversity preservation. The high warming rates allow much lower concentrations of cryoprotectants to be used, thereby reducing cryoprotectant toxicity and opening the way for a myriad of different biomaterials to be preserved and used in the future.
Ice formation during warming of zebrafish embryos is the most challenging barrier to successful cryopreservation. Research has shown that ice formation during rewarming can be minimized with high heating rates (up to14,000,000 Â°C/minute). Currently, no reproducible methods exist that can warm vitrified zebrafish embryos (and other similar sized cells or organisms) this rapidly or uniformly. A common warming method uses India ink (carbon black) as a laser absorber, but this ink is toxic when injected inside zebrafish embryos. The GNRs used in this technology are ten times more efficient for heatingâand heating uniformly across an embryoâwithout toxicity.
BENEFITS AND FEATURES:
Phase of Development - Proof of concept. Live zebrafish embryos, human dermal fibroblasts cells and coral larvae have been recovered using the process.
Microwave-assisted pyrolysis (MAP) maximizes yields by recovering methyl ester from biodiesel vacuum distillation bottoms (VDBs). The technology is a MAP reactor with a fixed-bed heat susceptor silicon carbide (SiC) catalyst that absorbs microwave radiation and quickly achieves a high temperature. The process rapidly heats VDBs, thus avoiding further dimerization and derivatization. After distillation microwave-assisted pyrolysis (dMAP), 85.9% wt/wt of the VDBs were recovered as a transparent bio-oil composed mostly of methyl esters. This bio-oil can be blended back into B100 biodiesel and certified for sale using ASTM D67514. Blending dMAP bio-oil (10% wt/wt) with B100 biodiesel meets all certification requirements and demonstrates that MAP processing could be a significant yield improvement technology for any commercial biodiesel producer.
Vacuum distillation in commercial-grade biodiesel production is a reliable post-treatment method for removing multiple impurities. However, biodiesel distillation, the most significant and primary purification process, produces a waste stream of VDBs that represents approximately 5-15% of the crude biodiesel. VDBs must either be disposed of or sold as low-grade heating oil for use in boilers or ocean shipping. This new MAP technology recovers valuable methyl esters from VDBs, solving the waste formation problem by recovering a significant amount (approximately 85% wt/wt) of the VDBs as a transparent bio-oil composed mostly of methyl esters. Testing shows the bio-oil can be blended back into the initial distillate stream and that it passes all ASTM D6751 tests required for commercial biodiesel. The process is easily integrated into existing biodiesel processes and can increase biodiesel yield, resulting in more biodiesel sold than low grade heating fuel.
Phase of Development - Proof of Concept
electrochemical method for ammonia synthesis by utilizing proton conducting,
anhydrous metal pyrophosphate/polymer composite membranes for protonation of
A method to synthesis ammonia using a lithium nitride-hydrogen iodide cycle.
An innovative transdermal
patch that diagnoses mild traumatic brain injury (MTBI).
NTIRE is a tissue ablation technology developed at the University of California for the ablation of tumor cells. Short electrical pulses are delivered, inducing irreparable nanoscale pores in the cell membrane, resulting in cell death. Studies have shown that the extracellular matrix and tissue architecture however remain intact. Researchers from UCSF have explored the potential use of NTIRE in the treatment of fibrotic diseases. Preliminary studies demonstrate that fibrotic tissues respond to NTIRE pulses differently than non-fibrotic tissue. It has also been shown that exogenous cells may be engrafted in-vivo into a host organ pre-treated with NTIRE. Therefore, post-NTIRE treated fibrotic tissue may be implanted with healthy cells to replace and regenerate healthy tissue and restore organ function.