简体中文
Register   |  Login  
   Switch to Russian AUTM's New Technology Profiles Thursday, August 16, 2018  
 AUTM's New Technology Profiles

Warming of Cryopreserved Biomaterials using Laser Assisted Gold Nanorods

Gold nanorods and coupled laser heating improves cryopreservation

A new platform technology uses laser assisted gold nanorods (GNRs) to warm cryopreserved zebrafish embryos, germplasm and other millimeter-sized tissues and biomaterials. Biocompatible gold nanorods absorb pulsed laser energy to generate rapid and uniform warming (up to 14,000,000 °C/min) in frozen biomaterials. Laser assisted GNR heating is the only method able to generate such high heating rates uniformly inside a millimeter-sized embryo, and it can also be used in cryopreserving a myriad of other germplasm, model systems, and other similar and smaller sized biomaterials into which GNR can be disbursed. This technology can positively impact translational medicine (which uses embryos as a genetic model of disease), embryology and toxicity as well as aquaculture and biodiversity preservation. The high warming rates allow much lower concentrations of cryoprotectants to be used, thereby reducing cryoprotectant toxicity and opening the way for a myriad of different biomaterials to be preserved and used in the future.

Increased efficiency, uniform of heating and low toxicity

Ice formation during warming of zebrafish embryos is the most challenging barrier to successful cryopreservation. Research has shown that ice formation during rewarming can be minimized with high heating rates (up to14,000,000 °C/minute). Currently, no reproducible methods exist that can warm vitrified zebrafish embryos (and other similar sized cells or organisms) this rapidly or uniformly. A common warming method uses India ink (carbon black) as a laser absorber, but this ink is toxic when injected inside zebrafish embryos. The GNRs used in this technology are ten times more efficient for heating—and heating uniformly across an embryo—without toxicity.

BENEFITS AND FEATURES:

  • Gold nanorod (GNRs)
  • Laser assisted gold GNR heating
  • Cryopreserved zebrafish embryos, germplasm, model systems and other millimeter-sized tissues and biomaterials
  • Rapid (up to14,000,000 °C/min) and uniform warming; faster speeds may be possible in the future
  • Lower concentrations of cryoprotectants needed
  • Non-toxic

APPLICATIONS:

  • Zebrafish embryos
  • Germplasm centers; germplasm stock centers and banking
  • Research
  • Biopsy preservation
  • Translational medicine (which uses embryos as a genetic model of disease)
  • Embryology and toxicity
  • Model systems
  • Aquaculture and biodiversity preservation

Phase of Development - Proof of concept. Live zebrafish embryos, human dermal fibroblasts cells and coral larvae have been recovered using the process.

Improving Biodiesel Production Efficiency via Methyl Ester Recovery

Methyl Ester Recovery via Microwave-Assisted Pyrolysis

Microwave-assisted pyrolysis (MAP) maximizes yields by recovering methyl ester from biodiesel vacuum distillation bottoms (VDBs). The technology is a MAP reactor with a fixed-bed heat susceptor silicon carbide (SiC) catalyst that absorbs microwave radiation and quickly achieves a high temperature. The process rapidly heats VDBs, thus avoiding further dimerization and derivatization. After distillation microwave-assisted pyrolysis (dMAP), 85.9% wt/wt of the VDBs were recovered as a transparent bio-oil composed mostly of methyl esters. This bio-oil can be blended back into B100 biodiesel and certified for sale using ASTM D67514. Blending dMAP bio-oil (10% wt/wt) with B100 biodiesel meets all certification requirements and demonstrates that MAP processing could be a significant yield improvement technology for any commercial biodiesel producer.

Solves Biodiesel Waste Formation Problems and Increases Yield

Vacuum distillation in commercial-grade biodiesel production is a reliable post-treatment method for removing multiple impurities. However, biodiesel distillation, the most significant and primary purification process, produces a waste stream of VDBs that represents approximately 5-15% of the crude biodiesel. VDBs must either be disposed of or sold as low-grade heating oil for use in boilers or ocean shipping. This new MAP technology recovers valuable methyl esters from VDBs, solving the waste formation problem by recovering a significant amount (approximately 85% wt/wt) of the VDBs as a transparent bio-oil composed mostly of methyl esters. Testing shows the bio-oil can be blended back into the initial distillate stream and that it passes all ASTM D6751 tests required for commercial biodiesel. The process is easily integrated into existing biodiesel processes and can increase biodiesel yield, resulting in more biodiesel sold than low grade heating fuel.

BENEFITS AND FEATURES:

  • Up to 10% increase in biodiesel yields for any commercial biodiesel producer
  • Decreases waste stream from biodiesel production
  • Recovers valuable methyl ester from biodiesel vacuum distillation bottoms (VDBs)
  • Employs energy efficient microwave-assisted pyrolysis (MAP)
  • Robust, cost effective, fixed-bed heat susceptor silicon carbide (SiC) catalyst
  • Minimizes dimerization and derivatization; rapid heating maximizes yield
  • Produces biodiesel that passes ASTM D6751 tests when blended into conventional bio-diesel
  • High temperatures required for biofuel distillation can be sustained

APPLICATIONS:

  • Biodiesel production and purification

Phase of Development - Proof of Concept

3-D PRINTED ORTHOPAEDIC IMPLANTS

Nonlinear Phononic Signal Processors

Method for the Electrochemical Synthesis of Ammonia Using a Metal Pyrophosphate–Polymer Composite Membrane

An electrochemical method for ammonia synthesis by utilizing proton conducting, anhydrous metal pyrophosphate/polymer composite membranes for protonation of nitrogen.

Method for the Electrochemical Synthesis of Ammonia Utilizing a Lithium Nitride-Hydrogen Iodide Cycle

A method to synthesis ammonia using a lithium nitride-hydrogen iodide cycle.

Mild Traumatic Brain Injury Diagnostic Microneedle Patch

An innovative transdermal patch that diagnoses mild traumatic brain injury (MTBI).

Thermally Regulating Paint


Thermally Regulating Paint


Using Non-Thermal Irreversible Electroporation To Induce Tissue Remodeling And Regeneration

NTIRE is a tissue ablation technology developed at the University of California for the ablation of tumor cells. Short electrical pulses are delivered, inducing irreparable nanoscale pores in the cell membrane, resulting in cell death. Studies have shown that the extracellular matrix and tissue architecture however remain intact. Researchers from UCSF have explored the potential use of NTIRE in the treatment of fibrotic diseases. Preliminary studies demonstrate that fibrotic tissues respond to NTIRE pulses differently than non-fibrotic tissue. It has also been shown that exogenous cells may be engrafted in-vivo into a host organ pre-treated with NTIRE. Therefore, post-NTIRE treated fibrotic tissue may be implanted with healthy cells to replace and regenerate healthy tissue and restore organ function.


    
© National Academy of Sciences of Belarus, Innovation Association "RCTT"
Besucherzahler meet and marry russian women
счетчик посещений
Рейтинг@Mail.ru Яндекс.Метрика